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Updated: Jul 24, 2025

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles "Nanoblades"
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Programmable RNA-guided endonucleases are widespread in eukaryotes and their viruses

Kaiyi Jiang1,2, Justin Lim1, Samantha Sgrizzi1

  • 1McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Biorxiv : the Preprint Server for Biology
|July 3, 2023
PubMed
Summary

Scientists discovered HERMES, novel RNA-guided nucleases in eukaryotes derived from prokaryotic TnpB proteins. These systems are adapted for eukaryotic cells and show potential for genome editing applications.

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