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Related Experiment Video

Updated: Jul 24, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

GENE EXPRESSION WITHIN A HUMAN CHOROIDAL NEOVASCULAR MEMBRANE USING SPATIAL TRANSCRIPTOMICS.

Andrew P Voigt^1,2, Nathaniel K Mullin^1,2, Emma M Navratil^1,2

¹Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242.

Biorxiv : the Preprint Server for Biology

|July 3, 2023

Summary

This summary is machine-generated.

This study used spatial RNA sequencing to analyze gene expression in macular neovascularization, identifying dysregulated genes in endothelial cells and potential upstream regulators like VEGF and TGFB1.

Keywords:

AMD choroid gene expression neovascularization spatial RNA sequencing

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Related Experiment Videos

Last Updated: Jul 24, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

Ophthalmology
Molecular Biology
Genomics

Background:

Macular neovascularization (MNV) is a key complication of age-related macular degeneration, leading to vision loss.
The cellular origins and molecular mechanisms driving MNV pathogenesis remain incompletely understood.
Pathologic angiogenesis in MNV can arise from choroidal or retinal sources.

Conclusions:

Spatial transcriptomics provides insights into cellular dysregulation in MNV.
Candidate genes and pathways identified may offer therapeutic targets for MNV.
Confirmed known regional gene expression patterns in ocular tissues.