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In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...

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Related Experiment Video

Updated: Jul 24, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

First babies conceived with Automated Intracytoplasmic Sperm Injection.

Nuno Costa-Borges¹, Santiago Munné², Eduard Albó³

¹Embryotools, Barcelona, Spain.

Reproductive Biomedicine Online

|July 3, 2023

Summary

This summary is machine-generated.

An automated sperm injection robot successfully performed Automated Intracytoplasmic Sperm Injection (ICSIA) in human IVF, achieving pregnancies and live births. This technology shows promise for assisted reproductive technologies.

Keywords:

AI ICSI ICSIA automation fertilization

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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

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Last Updated: Jul 24, 2025

Mouse Round Spermatid Injection

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Published on: January 26, 2024

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Published on: May 17, 2024

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Published on: October 5, 2016

Area of Science:

Reproductive biology
Robotics in medicine
Assisted reproductive technology

Background:

Intracytoplasmic Sperm Injection (ICSI) is a key technique in human IVF.
Automation of micromanipulation procedures in embryology is an emerging field.
The development of robotic systems aims to improve precision and consistency in assisted reproduction.

Purpose of the Study:

To evaluate the efficacy of an automated sperm injection robot for Automated Intracytoplasmic Sperm Injection (ICSIA).
To assess the feasibility of ICSIA in a clinical setting using human oocytes.
To compare the performance of the ICSIA robot with manual ICSI performed by experienced embryologists.

Main Methods:

The ICSIA robot automated sperm injection, including oocyte penetration and sperm release.
Testing was conducted on animal oocytes (mouse, hamster, rabbit) and discarded human oocytes.
A pilot clinical trial used donor oocytes, with the robot operated by engineers lacking micromanipulation experience.

Main Results:

The ICSIA robot showed comparable results to manual ICSI in animal models and pre-clinical human oocyte tests.
In clinical validation, ICSIA achieved a fertilization rate of 13/14 oocytes.
Pregnancies and live births were achieved following the transfer of ICSIA-derived blastocysts.

Conclusions:

The ICSIA robot demonstrated high proficiency in oocyte injection, even when operated by inexperienced personnel.
Preliminary clinical trial results indicate the robot's performance is within key indicators.
Automated ICSI holds potential for improving outcomes in human IVF.