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Yin Yang 1-Induced Long Noncoding RNA DUXAP9 Drives the Progression of Oral Squamous Cell Carcinoma by Blocking

Wenkai Zhou1,2, Yisheng Feng2, Chengzhong Lin2,3

  • 1Department of Oral and Maxillofacial & Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 4, 2023
PubMed

Insights

A novel long non-coding RNA, DUXAP9, promotes oral squamous cell carcinoma (OSCC) progression by stabilizing EZH2. Targeting DUXAP9 may offer a new therapeutic strategy for OSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are crucial in cancer, but their roles in oral squamous cell carcinoma (OSCC) are not fully elucidated.
  • Understanding lncRNA functions is vital for developing novel OSCC therapies.

Purpose of the Study:

  • To identify and characterize a novel lncRNA, DUXAP9, in OSCC.
  • To investigate the molecular mechanisms underlying DUXAP9's role in OSCC progression.
  • To evaluate DUXAP9 as a potential therapeutic target for OSCC.

Main Methods:

  • Identification and expression analysis of DUXAP9 in OSCC tissues and cell lines.
  • In vitro and in vivo experiments to assess the functional role of DUXAP9 in OSCC cell proliferation, migration, and invasion.
  • Investigation of DUXAP9's interaction with EZH2 and its downstream targets.
  • Analysis of the correlation between DUXAP9 expression and clinical parameters in OSCC patients.

Main Results:

  • DUXAP9 is highly expressed in OSCC and its high expression correlates with advanced clinical stage, lymph node metastasis, and poor patient survival.
  • Overexpression of DUXAP9 promotes OSCC cell proliferation, migration, invasion, and tumor growth, while its knockdown suppresses these processes.
  • DUXAP9 stabilizes EZH2 by inhibiting its phosphorylation and subsequent degradation, thereby promoting OSCC progression in an EZH2-dependent manner.
  • Yin Yang 1 (YY1) was identified as a transcriptional activator of DUXAP9.

Conclusions:

  • DUXAP9 is a novel oncogenic lncRNA that drives OSCC progression through the DUXAP9/EZH2 axis.
  • DUXAP9 expression levels are associated with poor prognosis in OSCC patients.
  • DUXAP9 represents a promising therapeutic target for oral squamous cell carcinoma.
Keywords:
EZH2LncRNA DUXAP9Yin Yang 1 (YY1)oral squamous cell carcinomaubiquitination

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