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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
Published on: January 7, 2019
Integrated mRNA and microRNA profiling in lung tissue and blood from human silicosis
Jingbo Zhang1, Weijiang Hu2, Kai Liu2
1Clinical Research Center of Occupational Diseases, The Affiliated Shanghai Pulmonary Hospital of Tongji University School of Medicine, Shanghai, China.
Background:
The overwhelming majority of subjects in the current silicosis mRNA and microRNA (miRNA) expression profile are of human blood, lung cells or a rat model, which puts limits on the understanding of silicosis pathogenesis and therapy. To address the limitations, our investigation was focused on differentially expressed mRNA and miRNA profiles in lung tissue from silicosis patients to explore potential biomarker for early detection of silicosis.
Methods:
A transcriptome study was conducted based on lung tissue from 15 silicosis patients and eight normal people, and blood samples from 404 silicosis patients and 177 normal people. Three early stage silicosis, five advanced silicosis and four normal lung tissues were randomly selected for microarray processing and analyze. The differentially expressed mRNAs were further used to conduct Gene Ontology and pathway analyses. Series test of cluster was performed to explore possible changes in differentially expressed mRNA and miRNA expression patterns during the process of silicosis. The blood samples and remaining lung tissues were used in a quantitative real-time PCR (RT-qPCR) (RT-qPCR).
Results:
In total, 1417 and 241 differentially expressed mRNAs and miRNAs were identified between lung tissue from silicosis patients and normal people (p < 0.05). However, there was no significant difference in most mRNA or miRNA expression between early stage and advanced stage silicosis lung tissues. RT-qPCR validation results in lung tissues showed expression of four mRNAs (HIF1A, SOCS3, GNAI3 and PTEN) and seven miRNAs was significantly down-regulated compared to those of control group. Nevertheless, PTEN and GNAI3 expression was significantly up-regulated (p < 0.001) in blood samples. The bisulfite sequencing PCR demonstrated that PTEN had significantly decreased the methylation rate in blood samples of silicosis patients.
Conclusions:
PTEN might be a potential biomarker for silicosis as a result of low methylation in the blood.
Insights
Researchers identified potential biomarkers for silicosis by analyzing gene expression in lung tissue and blood. PTEN, a gene with decreased methylation in silicosis patients
Area of Science:
- Pulmonary Medicine
- Molecular Biology
- Biomarker Discovery
Background:
- Current understanding of silicosis pathogenesis and therapy is limited by a focus on blood or rat models.
- Investigating gene expression profiles in lung tissue is crucial for advancing silicosis research.
- Identifying novel biomarkers is essential for early detection and improved treatment of silicosis.
Purpose of the Study:
- To identify differentially expressed messenger RNAs (mRNAs) and microRNAs (miRNAs) in lung tissue of silicosis patients.
- To explore potential biomarkers for the early detection of silicosis.
- To analyze gene and miRNA expression patterns during silicosis progression.
Main Methods:
- Transcriptome analysis of lung tissue from silicosis patients and healthy individuals.
- Microarray processing and analysis of selected lung tissue samples.
- Quantitative real-time PCR (RT-qPCR) and bisulfite sequencing PCR for validation in blood and lung tissues.
Main Results:
- 1417 differentially expressed mRNAs and 241 miRNAs were identified in lung tissue.
- PTEN mRNA and GNAI3 mRNA showed significant upregulation in silicosis patients' blood.
- Decreased methylation rate of PTEN was observed in blood samples from silicosis patients.
Conclusions:
- PTEN shows potential as a biomarker for silicosis due to its altered methylation in blood.
- Further research is warranted to validate PTEN as a reliable diagnostic marker for silicosis.
