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Related Experiment Videos

Developmental changes in the astrocytic response to lateral olfactory tract section.

H Sijbesma, C M Leonard

    The Anatomical Record
    |August 1, 1986
    PubMed
    Summary

    Axon regeneration in golden hamsters fails after postnatal day 7 due to developmental changes in the glial response. Greater astrocytic reactivity after P9 lesions hinders nerve regrowth, unlike earlier P3 lesions.

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    Area of Science:

    • Neuroscience
    • Developmental Biology
    • Regenerative Medicine

    Background:

    • Axon regeneration is crucial for functional recovery after nervous system injury.
    • The golden hamster's lateral olfactory tract (LOT) exhibits a critical developmental window for axonal regeneration.
    • Postnatal day 7 (P7) marks a transition from regenerative to non-regenerative capacity in the LOT.

    Purpose of the Study:

    • To investigate the role of astrocytic response in the developmental loss of LOT regeneration.
    • To compare glial reactions following LOT transection at different postnatal ages (P3 vs. P9).
    • To correlate changes in glial cells with the hamster's capacity for axonal regrowth.

    Main Methods:

    • Performed lateral olfactory tract (LOT) transections in golden hamsters at postnatal day 3 (P3) and P9.

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  • Utilized immunocytochemistry for glial fibrillary acidic protein (GFAP) to visualize astrocytic reactivity.
  • Observed glial response at survival times from 12 hours to 2 weeks post-lesion.

    • Main Results:

    • Significant glial reaction (astrocytic hypertrophy and process proliferation) observed at both P3 and P9.
    • Greater and more extensive astrocytic response occurred after P9 transections compared to P3.
    • Radial glial cells were present 2 weeks after P3 lesions but absent after P9 lesions.

    Conclusions:

    • Developmental maturation of the glial response, specifically astrocytes, underlies the loss of LOT regenerative capacity after P7.
    • The presence of radial glia after early lesions may create a permissive environment for axonal regeneration.
    • Understanding these glial changes could inform strategies to promote nerve repair in the mature nervous system.