Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regional differences in myocyte size in normal rat heart.

A M Gerdes, J A Moore, J M Hines

    The Anatomical Record
    |August 1, 1986
    PubMed
    Summary

    Reliable myocyte size measurements in adult rats were achieved using three methods. Significant regional differences in myocyte size exist, primarily due to variations in cross-sectional area, not cell length.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Laser-driven betatron x rays for high-throughput imaging of additively manufactured materials.

    The Review of scientific instruments·2024
    Same author

    Patient Experiences of Using Wearable Health Monitors During Cancer Treatment: A Qualitative Study.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2024
    Same author

    Surgery school-who, what, when, and how: results of a national survey of multidisciplinary teams delivering group preoperative education.

    Perioperative medicine (London, England)·2021
    Same author

    A healthy individual with a homozygous <i>PTCH2</i> frameshift variant: Are variants of <i>PTCH2</i> associated with nevoid basal cell carcinoma syndrome?

    Human genome variation·2019
    Same author

    Evaluation of tumor-infiltrating lymphocytes and association with prognosis in BRCA-mutated breast cancer.

    Acta oncologica (Stockholm, Sweden)·2019
    Same author

    Antibacterial activity of disulfiram and its metabolites.

    Journal of applied microbiology·2018

    Area of Science:

    • Cardiovascular Biology
    • Histology
    • Cell Biology

    Background:

    • Accurate measurement of cardiomyocyte size is crucial for understanding cardiac physiology and pathology.
    • Previous methods for assessing myocyte dimensions varied in accuracy and efficiency.

    Purpose of the Study:

    • To evaluate the reliability of three independent methods for determining adult rat cardiomyocyte size.
    • To compare the Coulter Channelyzer system with anatomical and morphometric techniques.
    • To investigate regional variations in cardiomyocyte size within the adult rat heart.

    Main Methods:

    • Cell volume determined using a Coulter Channelyzer system on isolated myocytes.
    • Cell volume calculated from the product of length and cross-sectional area of isolated myocytes.

    Related Experiment Videos

  • Myocyte cross-sectional area measured morphometrically from electron micrographs of perfusion-fixed tissue.

    • Main Results:

    • Myocyte dimensions obtained from all three techniques showed good agreement.
    • Significant differences in myocyte cross-sectional area and volume were observed between endomyocardium and epimyocardium of the left ventricle.
    • Right ventricle myocytes were smaller in volume and cross-sectional area compared to left ventricle myocytes, with no regional differences in cell length.

    Conclusions:

    • The Coulter Channelyzer system provides reliable myocyte volume values comparable to histometric techniques.
    • Isolated myocyte cross-sectional area measurements accurately represent values from whole-sectioned tissue.
    • Adult rat hearts exhibit significant regional variations in myocyte size, driven by differences in cross-sectional area.