Related Experiment Video For Cytology
Updated: Jul 24, 2025
Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
Published on: September 8, 2023
Whole Genome Sequencing in Advanced Lung Cancer can be Performed Using Diff-Quik Cytology Smears Derived from
David Fielding1,2, Andrew J Dalley3, Mahendra Singh3,4
1Department of Thoracic Medicine, The Royal Brisbane & Women's Hospital, Brisbane, Australia. David.fielding@health.qld.gov.au.
Introduction:
Maximising alternative sample types for genomics in advanced lung cancer is important because bronchoscopic samples may sometimes be insufficient for this purpose. Further, the clinical applications of comprehensive molecular analysis such as whole genome sequencing (WGS) are rapidly developing. Diff-Quik cytology smears from EBUS TBNA is an alternative source of DNA, but its feasibility for WGS has not been previously demonstrated.
Methods:
Diff-Quik smears were collected along with research cell pellets.
Results:
Tumour content of smears were compared to research cell pellets from 42 patients, which showed good correlation (Spearman correlation 0.85, P < 0.0001). A subset of eight smears underwent WGS, which presented similar mutation profiles to WGS of the matched cell pellet. DNA yield was predicted using a regression equation of the smears cytology features, which correctly predicted DNA yield > 1500 ng in 7 out of 8 smears.
Conclusions:
WGS of commonly collected Diff-Quik slides is feasible and their DNA yield can be predicted.
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