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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
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Global and tissue-specific aging effects on murine proteomes
Gregory R Keele1, Ji-Gang Zhang1, John Szpyt2
1The Jackson Laboratory, Bar Harbor, ME 04609, USA.
Cell Reports
|July 5, 2023
Summary
Aging degrades protein homeostasis, leading to decline and disease. This study reveals age-related protein changes across 10 tissues, showing immune infiltration and altered protein complex stoichiometry.
Area of Science:
- Proteomics and aging research
- Molecular biology of aging
- Systems biology of aging
Background:
- Protein homeostasis is crucial for health and declines with age.
- Previous aging studies focused on gene expression (transcriptomics).
- Understanding protein-level changes is vital for aging research.
Purpose of the Study:
- To investigate age-related changes directly at the protein level.
- To identify tissue-specific protein alterations during aging.
- To explore the impact of aging on protein complex stoichiometry.
Main Methods:
- Discovery-based proteomics was employed.
- Samples included 10 tissues from male and female C57BL/6J mice at 8 and 18 months of age.
- Quantitative proteomic analysis was performed.
Main Results:
- Age-related protein abundance changes were observed, often without corresponding transcriptional changes.
- A global increase in immune proteins was detected across all tissues.
- Tissue-specific alterations in endoplasmic reticulum and protein trafficking were identified in the spleen.
- Changes in the stoichiometry of key protein complexes, including CCT/TriC and the large ribosomal subunit, were noted.
Conclusions:
- Proteins play a significant role in systemic aging across multiple tissues.
- Age-related protein alterations contribute to functional decline and disease.
- This proteomic dataset provides a foundation for future aging research.
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