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Related Experiment Video

Updated: Jul 24, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Global and tissue-specific aging effects on murine proteomes.

Gregory R Keele¹, Ji-Gang Zhang¹, John Szpyt²

¹The Jackson Laboratory, Bar Harbor, ME 04609, USA.

|July 5, 2023

Summary

Aging degrades protein homeostasis, leading to decline and disease. This study reveals age-related protein changes across 10 tissues, showing immune infiltration and altered protein complex stoichiometry.

Keywords:

B6 C57BL/6J CP: Genomics CP: Metabolism TMT multitissue organismal aging protein complex protein homeostasis proteomics proteostasis tandem mass tag

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Area of Science:

Proteomics and aging research
Molecular biology of aging
Systems biology of aging

Background:

Protein homeostasis is crucial for health and declines with age.
Previous aging studies focused on gene expression (transcriptomics).
Understanding protein-level changes is vital for aging research.

Purpose of the Study:

To investigate age-related changes directly at the protein level.
To identify tissue-specific protein alterations during aging.
To explore the impact of aging on protein complex stoichiometry.

Main Methods:

Discovery-based proteomics was employed.
Samples included 10 tissues from male and female C57BL/6J mice at 8 and 18 months of age.
Quantitative proteomic analysis was performed.

Main Results:

Age-related protein abundance changes were observed, often without corresponding transcriptional changes.
A global increase in immune proteins was detected across all tissues.
Tissue-specific alterations in endoplasmic reticulum and protein trafficking were identified in the spleen.
Changes in the stoichiometry of key protein complexes, including CCT/TriC and the large ribosomal subunit, were noted.

Conclusions:

Proteins play a significant role in systemic aging across multiple tissues.
Age-related protein alterations contribute to functional decline and disease.
This proteomic dataset provides a foundation for future aging research.