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Updated: Jul 24, 2025

Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
Heritable epigenetic modification of BpPIN1 is associated with leaf shapes in Betula pendula
Chenrui Gu1, Rui Han1, Chaoyi Liu1
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, No. 51, Hexing Road, Harbin, Heilongjiang 150040, China.
Abstract:
The new variety Betula pendula 'Dalecarlica', selected from Betula pendula, shows high ornamental value owing to its lobed leaf shape. In this study, to identify the genetic components of leaf shape formation, we performed bulked segregant analysis and molecular marker-based fine mapping to identify the causal gene responsible for lobed leaves in B. pendula 'Dalecarlica'. The most significant variations associated with leaf shape were identified within the gene BpPIN1 encoding a member of the PIN-FORMED family, responsible for the auxin efflux carrier. We further confirmed the hypomethylation at the promoter region promoting the expression level of BpPIN1, which causes stronger and longer veins and lobed leaf shape in B. pendula 'Dalecarlica'. These results indicated that DNA methylation at the BpPIN1 promoter region is associated with leaf shapes in B. pendula. Our findings revealed an epigenetic mechanism of BpPIN1 in the regulation of leaf shape in Betula Linn. (birch), which could help in the molecular breeding of ornamental traits.
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