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First transcriptome profiling in gill and hepatopancrease tissues of Metapenaeus ensis in response to acute ammonia-N

Yun-Dong Li1, Meng-Ru Si2, Shi-Gui Jiang2

  • 1Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China; Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Hainan Aquaculture Breeding Engineering Research Center, College of Marine Sciences, Hainan University, Haikou, 570228, China; Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya Tropical Fisheries Research Institute, Sanya, 572018, China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518121, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572025, China.

Fish & Shellfish Immunology
|July 5, 2023
PubMed
Summary

Greasyback shrimp (Metapenaeus ensis) face ammonia-N stress in aquaculture. This study identified genes involved in their response, revealing mechanisms for improved stress tolerance and breeding strategies.

Keywords:
Adaptive strategiesAmmonia-N stressMetabolic mechanismsMetapenaeus ensisTranscriptome

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Area of Science:

  • Aquaculture
  • Genomics
  • Environmental Stress Biology

Background:

  • Intensive aquaculture of greasyback shrimp (Metapenaeus ensis) is hampered by ammonia-N toxicity.
  • Understanding the molecular mechanisms of ammonia-N stress tolerance is crucial for M. ensis breeding and aquaculture optimization.

Purpose of the Study:

  • To identify genes and genetic networks involved in the adaptive response of M. ensis to ammonia-N stress.
  • To explore potential molecular indicators for ammonia-N stress in M. ensis.

Main Methods:

  • Transcriptome sequencing and analysis of M. ensis under high ammonia-N conditions.
  • Identification of differentially expressed genes (DEGs) in gill and hepatopancreas tissues.
  • Assembly and clustering of unigenes to build genetic networks.

Main Results:

  • Over 100,000 unigenes were assembled, with thousands of DEGs identified in gill and hepatopancreas.
  • DEGs are significantly associated with immune function, oxidative stress, metabolism, and apoptosis.
  • M. ensis appears to counteract ammonia-N toxicity by upregulating immune and detoxification genes.

Conclusions:

  • The study elucidates the transcriptome-level adaptation of M. ensis to ammonia-N toxicity.
  • Identified genes can serve as molecular markers for ammonia-N stress and targets for selective breeding.
  • Findings support advancements in M. ensis breeding and factory aquaculture practices.