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Cellular senescence implication in mustard keratopathy
Mohammad Soleimani1, Seyed Mahbod Baharnoori1, Kasra Cheraqpour2
1Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USA; Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Experimental Eye Research
|July 5, 2023
Summary
Nitrogen mustard exposure causes cellular senescence in the cornea, leading to delayed-onset mustard keratopathy. Targeting senescence may offer new treatments for corneal damage and fibrosis.
Area of Science:
- Ophthalmology
- Toxicology
- Cellular Biology
Background:
- Mustard agents are chemical warfare vesicants causing significant ocular surface damage.
- Mustard keratopathy (MK) presents as corneal opacification, ulceration, and neovascularization, potentially leading to vision loss.
- Emerging evidence suggests cellular senescence plays a role in mustard-induced injuries and impaired wound healing.
Purpose of the Study:
- To establish the first murine model of delayed-onset mustard keratopathy (MK).
- To investigate the role of nitrogen mustard-induced cellular senescence in the cornea.
- To evaluate the correlation between senescence markers and corneal fibrosis.
Main Methods:
- Developed a murine model for delayed-onset MK using nitrogen mustard.
- Assessed pathological signs of senescence in corneal cells via beta-galactosidase staining.
- Quantified the relationship between beta-galactosidase staining and corneal fibrosis.
Main Results:
- Nitrogen mustard exposure induced cellular senescence in corneal cells.
- A significant correlation was observed between beta-galactosidase staining and the degree of corneal fibrosis.
- Senescence appears to be an underlying mechanism for chronic and late-onset ocular surface damage.
Conclusions:
- Nitrogen mustard exposure induces corneal cell senescence, contributing to delayed-onset MK.
- Cellular senescence is a key pathological mechanism in mustard-induced corneal injury.
- Anti-senescence therapies hold promise for accelerating corneal healing and reducing fibrosis in MK patients.
Keywords:
AgingBeta-galactosidaseMustard gasMustard keratopathyNitrogen mustardOcular surfaceSenescenceMore Related Videos
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