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Related Concept Videos

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Related Experiment Video

Updated: Jul 24, 2025

Dependence of Laser-induced Breakdown Spectroscopy Results on Pulse Energies and Timing Parameters Using Soil Simulants
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Phosphorus quantification in soil using LIBS assisted by laser-induced fluorescence.

Shweta Soni1,2, Jan Viljanen1, Risto Uusitalo3

  • 1Photonics Laboratory, Physics Unit, Tampere University, FI-33101, Tampere, Finland.

Heliyon
|July 6, 2023
PubMed
Summary

Accurate soil phosphorus monitoring is vital for agriculture and preventing eutrophication. Laser-induced breakdown spectroscopy with fluorescence (LIBS-LIF) offers improved detection limits for soluble phosphorus, reducing lab work.

Keywords:
DiagnosticsFluorescenceLIBSPhosphorusSoil

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Area of Science:

  • Agricultural Science
  • Analytical Chemistry
  • Environmental Science

Background:

  • Soil phosphorus quantification is crucial for sustainable agriculture and preventing water eutrophication.
  • Phosphorus deficiency negatively impacts crop development and growth.
  • Accurate monitoring of soil phosphorus is essential for optimal agricultural practices.

Purpose of the Study:

  • To introduce laser-induced breakdown spectroscopy assisted by laser-induced fluorescence (LIBS-LIF) for quantifying readily soluble phosphorus in soil.
  • To compare the analytical performance of LIBS-LIF with the conventional laser-induced breakdown spectroscopy (LIBS) method.
  • To evaluate the detection limits and potential for high-throughput analysis of soluble phosphorus in different soil types.

Main Methods:

  • Utilized laser-induced breakdown spectroscopy assisted by laser-induced fluorescence (LIBS-LIF) for soil analysis.
  • Employed mineral soils with varying phosphorus levels for method development and validation.
  • Generated calibration curves to determine the detection limits for soluble phosphorus.

Main Results:

  • LIBS-LIF significantly improved the detection limit for soluble phosphorus compared to conventional LIBS.
  • Detection limits for LIBS-LIF were 0.12 mg/kg for clay soil and 0.27 mg/kg for silt loam/loam soil.
  • The achieved detection limits with LIBS-LIF are comparable to established chemical soil analyses.

Conclusions:

  • LIBS-LIF offers a more sensitive method for quantifying soluble soil phosphorus.
  • This technique substantially reduces sample preparation and laboratory workload compared to traditional methods.
  • LIBS-LIF shows potential for high-throughput soil analysis, with calibrations holding within specific soil types.