Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

391
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
391
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

829
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
829
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

1.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.1K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

946
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
946

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving Speed and Efficiency of DESI Imaging with the Xevo MRT Mass Spectrometer for Analyte Mapping.

Metabolites·2026
Same author

Modulation of pain by affective touch: effects of childhood trauma, depression, chronic pain, and spinal segment.

The Journal of physiology·2026
Same author

Mass spectrometry imaging of lipids in a gut epithelial cell model.

The Analyst·2025
Same author

Systematic method optimisation approach for small molecule imaging on the SELECT SERIES MALDI MRT - Drug mapping in fingerprints, a case study.

Analytica chimica acta·2025
Same author

Visualizing active fungicide formulation mobility in tomato leaves with desorption electrospray ionisation mass spectrometry imaging.

The Analyst·2024
Same author

MALDI versus DESI mass spectrometry imaging of lipids in atherosclerotic plaque.

Rapid communications in mass spectrometry : RCM·2024

Related Experiment Video

Updated: Jul 24, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K

Update DESI Mass Spectrometry Imaging (MSI).

Emmanuelle Claude1, Mark Towers2, Emrys Jones2

  • 1Waters Corporation, Wilmslow, UK. Emmanuelle_Claude@waters.com.

Methods in Molecular Biology (Clifton, N.J.)
|July 6, 2023
PubMed
Summary
This summary is machine-generated.

High-sensitivity desorption electrospray ionization mass spectrometry imaging (DESI-MSI) now achieves low-micrometer pixel sizes for analyzing metabolites and lipids in biological tissues. This advancement enhances DESI-MSI

Keywords:
AmbientCo-registrationDESIHeated transfer lineHigh definition imaging

More Related Videos

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
08:02

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Published on: December 16, 2022

1.9K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.5K

Related Experiment Videos

Last Updated: Jul 24, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

18.7K
Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI
08:02

Visualization of Metabolites Identified in the Spatial Metabolome of Traditional Chinese Medicine Using DESI-MSI

Published on: December 16, 2022

1.9K
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI

Published on: March 24, 2016

9.5K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Desorption electrospray ionization (DESI) is an ambient technique for direct chemical analysis of surfaces without pretreatment.
  • Recent advancements have focused on improving DESI's capabilities for mass spectrometry imaging (MSI).

Purpose of the Study:

  • To describe improvements in DESI-MSI for high-sensitivity analysis of metabolites and lipids in biological tissue sections.
  • To achieve low-micrometer pixel sizes in DESI-MSI experiments.

Main Methods:

  • Utilized improved DESI source and coupled mass spectrometer configurations.
  • Developed methods for high-sensitivity metabolite and lipid detection in tissue sections.

Main Results:

  • Achieved low tens of microns pixel size for MSI experiments.
  • Demonstrated high sensitivity for detecting metabolites and lipids from biological tissue sections.

Conclusions:

  • DESI-MSI has undergone significant developmental improvements over the last decade.
  • DESI-MSI is emerging as a competitive technique to matrix-assisted laser desorption/ionization (MALDI) for mass spectrometry imaging.