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Updated: Jul 24, 2025
Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Liquid Extraction Surface Analysis Mass Spectrometry Imaging of Denatured Intact Proteins
Emma K Sisley1, James W Hughes1, Oliver J Hale1
1School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK.
Abstract:
Liquid extraction surface analysis (LESA) is an ambient surface sampling technique that can be coupled with mass spectrometry (MS) to analyze analytes directly from biological substrates such as tissue sections. LESA MS involves liquid microjunction sampling of a substrate by use of a discrete volume of solvent followed by nano-electrospray ionization. As the technique makes use of electrospray ionization, it lends itself to the analysis of intact proteins. Here, we describe the use of LESA MS to analyze and image the distribution of intact denatured proteins from thin fresh frozen tissue sections.
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