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Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization

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Enhancing Intracellular Optical Performance and Stability of Engineered Nanomaterials via Aqueous Two-Phase

Aceer Nadeem¹, Aidan Kindopp¹, Ian Wyllie¹

¹Department of Chemical Engineering, University of Rhode Island, Kingston, Rhode Island 02881, United States.

|July 6, 2023

Summary

Aqueous two-phase (ATP) purification enhances DNA-single-walled carbon nanotube (SWCNT) biosensors. Purified DNA-SWCNTs show improved optical properties and longer cellular retention, advancing biosensor development.

Keywords:

aqueous two-phase purification biosensing nanoparticle exocytosis nanoparticle optical stability near-infrared fluorescence

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Area of Science:

Nanomaterials Science
Biomedical Engineering
Optical Spectroscopy

Background:

Supramolecular hybrids of DNA and single-walled carbon nanotubes (SWCNTs) are vital in biosensing due to their optical characteristics.
Aqueous two-phase (ATP) purification methods are increasingly used for SWCNTs, improving specificity and homogeneity in sensor design.

Purpose of the Study:

To investigate the impact of ATP purification on DNA-SWCNT cellular retention and optical performance.
To evaluate the stability and fluorescence intensity of ATP-purified DNA-SWCNTs within murine macrophages over time.

Main Methods:

Utilized murine macrophages as a cellular model.
Employed near-infrared and Raman microscopy for probing.
Compared ATP-purified DNA-SWCNTs with as-dispersed SWCNTs over a 6-hour period.

Main Results:

ATP purification significantly increased the retention time of DNA-SWCNTs within cells.
Observed a 45% increase in fluorescence intensity for ATP-purified DNA-SWCNTs.
No significant change in emission wavelength was detected, indicating enhanced optical stability.

Conclusions:

Cellular processing of engineered nanomaterials is dependent on their purification state.
ATP purification enhances the optical performance and cellular stability of DNA-SWCNTs.
This work supports the development of more robust and sensitive biosensors with improved in vivo optical parameters.