Related Experiment Video
Updated: Jul 24, 2025
07:10
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
9.0K
Interplay between human STING genotype and bacterial NADase activity regulates inter-individual disease variability.
Elin Movert1, Jaume Salgado Bolarin1, Christine Valfridsson1
1Department of Biology, Lund University, Sölvegatan 35, 223 62, Lund, Sweden.
Nature Communications
|July 6, 2023
Summary
Host STING genotype and Streptococcus pyogenes NADase activity determine invasive infection outcomes. Reduced STING binding plus high bacterial NADase activity leads to severe disease, while robust STING response offers protection.
Area of Science:
- Microbiology
- Immunology
- Genetics
Background:
- Invasive Streptococcus pyogenes infections exhibit variable severity due to host-pathogen genetic interactions.
- The STING pathway and type I Interferon (IFN) response are crucial in innate immunity against bacterial pathogens.
Purpose of the Study:
- To investigate the interplay between human STING genotype and bacterial NADase activity in regulating invasive Streptococcus pyogenes infection outcomes.
- To elucidate the mechanism by which bacterial NADase influences the host immune response.
Main Methods:
- Analysis of Streptococcus pyogenes-derived cyclic di-AMP (c-di-AMP) diffusion through streptolysin O pores.
- Assessment of STING activation and type I IFN production in macrophages.
- Genotyping of STING in patients with necrotizing S. pyogenes infections.
- Correlation of bacterial NADase activity with patient outcomes.
Main Results:
- Bacterial c-di-AMP activates STING and type I IFN response via streptolysin O pores.
- Bacterial NADase activity suppresses STING-mediated type I IFN production.
- A specific STING genotype with reduced c-di-AMP binding capacity, coupled with high bacterial NADase activity, correlates with poor patient outcomes in necrotizing infections.
Conclusions:
- Bacterial NADase possesses an immune-regulatory function, suppressing host type I IFN responses.
- The interaction between host STING genotype and bacterial NADase activity is a key determinant of invasive Streptococcus pyogenes infection severity and interindividual variability.
- Understanding this host-pathogen genotype interplay offers insights into disease pathogenesis and potential therapeutic targets.
More Related Videos
Related Concept Videos
02:32Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
01:28Gene Regulation in Microbial Communities: Quorum Sensing
43
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
43
01:29Modern Molecular Taxonomy
54
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
54
01:16Transduction
46
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
46