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Long non‑coding RNA PTCSC3 suppresses triple‑negative breast cancer by downregulating long non‑coding RNA MIR100HG

Guojun Zhang1, Lei Gao1, Junliang Zhang1

  • 1Department of General Surgery, Changle People's Hospital, Changle County, Shandong 262499, P.R. China.

Oncology Letters
|July 7, 2023
PubMed

Insights

Long non-coding RNA (lncRNA) PTCSC3 acts as a tumor suppressor in triple-negative breast cancer (TNBC). It inhibits cancer cell viability and promotes apoptosis by downregulating MIR100HG, impacting patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) PTCSC3 is a known tumor suppressor in thyroid cancer and glioma.
  • Its role in triple-negative breast cancer (TNBC) remains largely unexplored.

Purpose of the Study:

  • To investigate the function and molecular mechanisms of PTCSC3 in triple-negative breast cancer (TNBC).
  • To explore the relationship between PTCSC3, MIR100HG, and TNBC progression.

Main Methods:

  • Analysis of PTCSC3 and MIR100HG expression in 82 TNBC patient tissues.
  • Cell viability (CCK-8) and apoptosis (Annexin V-FITC) assays.
  • Western blot analysis to investigate signaling pathways (Hippo pathway).

Main Results:

  • PTCSC3 was downregulated, and MIR100HG was upregulated in TNBC tissues.
  • Low PTCSC3 and high MIR100HG expression correlated with poor patient survival.
  • PTCSC3 overexpression inhibited MIR100HG, suppressed TNBC cell viability, and promoted apoptosis via the Hippo pathway.
  • MIR100HG overexpression promoted TNBC cell viability and inhibited apoptosis, counteracting PTCSC3 effects.

Conclusions:

  • lncRNA PTCSC3 functions as a tumor suppressor in TNBC by downregulating MIR100HG.
  • PTCSC3 inhibits TNBC cell viability and promotes apoptosis, potentially through the Hippo signaling pathway.
  • PTCSC3 and MIR100HG are significant prognostic biomarkers for TNBC patients.
Keywords:
apoptosislong non-coding RNA MIR100HGlong non-coding RNA PTCSC3triple-negative breast cancerviability

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