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FABP5 suppresses colorectal cancer progression via mTOR-mediated autophagy by decreasing FASN expression

Mujie Ye1, Chunhua Hu1, Tiaotiao Chen1

  • 1Department of Geriatric Gastroenterology, Neuroendocrine Tumor Center, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Institute of Neuroendocrine Tumor, Nanjing Medical University, Nanjing, China.

International Journal of Biological Sciences
|July 7, 2023
PubMed

Insights

Fatty acid-binding protein 5 (FABP5) suppresses colorectal cancer (CRC) progression by inhibiting proliferation and tumor growth. Its downregulation in CRC suggests FABP5 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lipid metabolism is crucial in cancer development, particularly in digestive system tumors like colorectal cancer (CRC).
  • Fatty acid-binding protein 5 (FABP5) role in CRC remains to be fully elucidated.

Purpose of the Study:

  • Investigate the function of FABP5 in colorectal cancer (CRC).
  • Elucidate the underlying molecular mechanisms of FABP5's action in CRC.
  • Identify potential therapeutic targets for CRC intervention.

Main Methods:

  • Gene expression analysis in CRC tissues.
  • In vitro functional assays (proliferation, colony formation, migration, invasion).
  • In vivo tumor growth studies.
  • Mechanistic studies involving protein-protein interactions, ubiquitination assays, and signaling pathway analysis.
  • Pharmacological inhibition of FASN.

Main Results:

  • FABP5 was significantly downregulated in CRC.
  • FABP5 exhibited inhibitory effects on CRC cell proliferation, colony formation, migration, invasion, and tumor growth.
  • FABP5 interacted with FASN, promoting its degradation via the ubiquitin-proteasome pathway, reducing lipid accumulation, suppressing mTOR signaling, and enhancing autophagy.
  • Orlistat (FASN inhibitor) demonstrated anti-cancer effects.
  • ALKBH5 positively regulated FABP5 expression through an m6A-independent mechanism.

Conclusions:

  • FABP5 acts as a tumor suppressor in CRC.
  • The ALKBH5/FABP5/FASN/mTOR axis is critical in CRC progression.
  • Targeting this axis offers potential therapeutic strategies for CRC.
Keywords:
FASNAutophagyColorectal cancerFABP5Lipid metabolismN6-methyladenosineOrlistat

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