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Updated: Jul 24, 2025

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Deploying solid-phase synthesis to access thymine-containing nucleoside analogs that inhibit DNA repair nuclease
Christine A Arbour1, Ellen M Fay2, Joanna F McGouran2
1Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. imper@mit.edu.
Abstract:
Nucleoside analogs show useful bioactive properties. A versatile solid-phase synthesis that readily enables the diversification of thymine-containing nucleoside analogs is presented. The utility of the approach is demonstrated with the preparation of a library of compounds for analysis with SNM1A, a DNA damage repair enzyme that contributes to cytotoxicity. This exploration provided the most promising nucleoside-derived inhibitor of SNM1A to date with an IC50 of 12.3 μM.
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