Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pigmentation01:19

Pigmentation

2.5K
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
2.5K
Mutations01:35

Mutations

38.3K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
38.3K
Skin Cancer01:30

Skin Cancer

4.2K
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
4.2K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.9K
Role of Skin in Vitamin D Synthesis01:23

Role of Skin in Vitamin D Synthesis

5.3K
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin...
5.3K
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Operative Duration as an Independent Risk Factor for Lower-Extremity Deep Vein Thrombosis Following Shoulder Arthroscopy: A Prospective Cohort Study With Systematic Ultrasonographic Screening.

Orthopaedic journal of sports medicine·2026
Same author

Structural evolution of lotus seed resistant starch during <i>in vitro</i> fecal fermentation in food-allergic rats modulates gut microbiota and SCFAs.

Frontiers in nutrition·2026
Same author

Investigating the Gut Microbiota-Inflammatory Cytokine-Skin Axis in Inflammatory Skin Diseases: Evidence from Mendelian Randomization.

Clinical, cosmetic and investigational dermatology·2026
Same author

Corrigendum to "In vitro assessment of the effect of Porphyra haitanensis polysaccharides on the intestinal flora of allergic mice" [Int. J. Biol. Macromol. 311 (2025) 143950].

International journal of biological macromolecules·2026
Same author

Direct air capture technologies: innovations, integration, and pathways to scale.

Chemical Society reviews·2026
Same author

Lotus seed resistant starch alleviates OVA-induced food allergy in rats by promoting a Bifidobacterium-enriched gut microbiota and enhancing acetic acid production.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: Jul 24, 2025

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
12:37

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Published on: September 7, 2013

18.3K

UVB promotes melanogenesis by regulating METTL3.

Haoran Guo1, Hongliang Zeng2, Yibo Hu1

  • 1Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, China.

Journal of Cellular Physiology
|July 7, 2023
PubMed
Summary

Ultraviolet (UV) radiation boosts skin pigment production via N6-methyladenosine (m6 A) modification. This epigenetic process, regulated by METTL3, increases YAP1 expression, promoting melanogenesis.

Keywords:
METTL3N6-methyladenineYAPmelanogenesisultraviolet

More Related Videos

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
06:09

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

8.8K
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
07:16

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish

Published on: March 1, 2022

2.3K

Related Experiment Videos

Last Updated: Jul 24, 2025

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
12:37

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Published on: September 7, 2013

18.3K
Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
06:09

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

8.8K
Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish
07:16

Reverse Genetic Approach to Identify Regulators of Pigmentation using Zebrafish

Published on: March 1, 2022

2.3K

Area of Science:

  • Epigenetics
  • Dermatology
  • Molecular Biology

Background:

  • Ultraviolet (UV) radiation is a primary external factor inducing skin pigmentation.
  • The precise mechanisms of UV-induced melanogenesis, particularly epigenetic regulation, require further elucidation.
  • N6-methyladenosine (m6 A) modification is a key epigenetic mechanism influencing diverse biological processes.

Purpose of the Study:

  • To investigate the role and mechanisms of m6 A modification in UV radiation (UVB)-induced melanogenesis.
  • To explore the relationship between METTL3, YAP1, and melanogenesis under UVB exposure.

Main Methods:

  • Analysis of global m6 A levels in melanocytes and MNT1 cells after UVB irradiation.
  • Gene expression analysis of METTL3 and melanogenesis-related genes.
  • Overexpression and knockdown of METTL3 in MNT1 cells.
  • Assessment of YAP1 protein levels and m6 A modification sites.
  • Inhibition of YAP1 expression to evaluate its role in METTL3-induced melanogenesis.

Main Results:

  • Low-dose UVB increased global m6 A modification in melanocytes and MNT1 cells.
  • METTL3 expression positively correlated with MITF in sun-exposed skin.
  • METTL3 overexpression upregulated melanin content and melanogenesis genes, while knockdown downregulated them, effects amplified by UVB.
  • METTL3 modulated YAP1 protein levels, with three predicted m6 A sites on YAP1 mRNA confirmed.
  • YAP1 inhibition partially reversed METTL3-induced melanogenesis.

Conclusions:

  • UVB irradiation enhances global m6 A modification in melanocytes.
  • METTL3 is upregulated by UVB and promotes melanogenesis by increasing YAP1 expression via m6 A modification.
  • This pathway, involving METTL3, YAP1, and TEAD1, plays a significant role in UVB-induced melanogenesis.