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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published on: May 1, 2019
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METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity
Chen Li1, Xiaoyan Wu1,2, Ying Yang2
1Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. China.
Virulence
|July 7, 2023
Summary
N6-methyladenosine (m6A) methylation positively impacts Porcine circovirus type 2 (PCV2) replication. Targeting methyltransferase METTL14 and demethylase FTO offers new strategies for PCV2 prevention and control.
Area of Science:
- Epigenetics
- Virology
- Molecular Biology
Background:
- N6-methyladenosine (m6A) methylation is crucial in viral infections.
- The role of m6A in Porcine circovirus type 2 (PCV2) replication remains largely uncharacterized.
Purpose of the Study:
- To investigate the role of m6A modification in PCV2 replication.
- To elucidate the mechanisms by which m6A affects PCV2 virus reproduction.
Main Methods:
- PK-15 cells were infected with PCV2.
- Expression levels of METTL14 and FTO were analyzed.
- m6A methylation levels were assessed following manipulation of METTL14 and FTO.
- miRNA maturity, specifically miRNA-30a-5p, was evaluated.
Main Results:
- PCV2 infection increased m6A levels in PK-15 cells.
- PCV2 infection upregulated the expression of METTL14 and FTO.
- Inhibition of METTL14 reduced m6A levels and PCV2 replication.
- Depletion of FTO increased m6A levels and stimulated PCV2 replication.
- METTL14 and FTO were found to regulate PCV2 replication via miRNA-30a-5p maturation.
Conclusions:
- m6A modification positively regulates PCV2 replication.
- METTL14 and FTO are key players in PCV2 replication through epigenetic mechanisms.
- Understanding the m6A pathway offers novel avenues for PCV2 control.
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