Radioimmunoassay of LH-RH. Application to human plasma

Comparison of standard curves with different concentrations of human plasma in the medium of incubation is presented. It appears that Bo varies with the dilution although the ratio B/Bo is independent of the dilution. This last observation was used to research 'endogenous' LH-RH in four menstrual cycles, in postmenopausal women and in men. The validity of the dosage is discussed in a more general way in the light of the results published in the literature.