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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Editorial for the Special Issue on Micromachines for Dielectrophoresis, Volume II

Rodrigo Martinez-Duarte1

  • 1Multiscale Manufacturing Laboratory, Department of Mechanical Engineering, Clemson University, Clemson, SC 29634, USA.

Micromachines
|July 8, 2023
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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...