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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

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Microgel-based etalon immunoassay for IgG detection.

Qiming Shen¹, Changhao Fang¹, Michael J Serpe²

¹Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.

Analytical and Bioanalytical Chemistry

|July 8, 2023

Summary

We created a new immunoassay for quantifying mouse immunoglobulin G (IgG) using microgel-based etalon devices. This method offers sensitive detection of mouse IgG, crucial for various biological studies.

Keywords:

Etalon HRP Immunoassay Mouse IgG detection pNIPAm-co-AAc microgel

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Area of Science:

Biochemistry
Materials Science
Analytical Chemistry

Background:

Accurate quantitation of mouse immunoglobulin G (IgG) is essential for immunological research.
Existing methods may have limitations in sensitivity or require complex procedures.
Development of novel, sensitive, and efficient immunoassay platforms is ongoing.

Purpose of the Study:

To develop and validate a novel immunoassay for sensitive mouse IgG quantitation.
To utilize poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgel-based etalon devices for this purpose.

Main Methods:

Immobilization of biotinylated anti-mouse IgG antibody onto a streptavidin-modified etalon surface.
Capture of mouse IgG from solution.
Detection using HRP-conjugated secondary antibody and 4-chloro-1-naphthol (4CN) substrate.
Quantification of 4CN concentration change via etalon reflectance peak shift.

Main Results:

The developed etalon-based immunoassay successfully quantified mouse IgG.
The assay demonstrated high sensitivity, detecting mouse IgG down to 0.018 nM.
A linear detection range of 0.02-5 nM for mouse IgG was established.

Conclusions:

The pNIPAm-co-AAc microgel-based etalon device provides a sensitive platform for mouse IgG quantitation.
This novel immunoassay offers a promising tool for immunological research and diagnostics.
The method's sensitivity and linear range support its utility in detecting low concentrations of mouse IgG.