Transient expression of neurofilament-like (RT97) immunoreactivity in cerebellar granule cells

The expression of the 200-kDa neurofilament subunit in developing rat cerebellar granule cells in vivo and in vitro was examined by immunocytochemistry and immunoblotting. Granule cells in vitro were found to express neurofilament-like immunoreactivity. In cryostat sections, neurofilament immunoreactivity was evident between P6 and P13 as a band of staining in the molecular layer indicative of staining of parallel fibres. No labelling of parallel fibres was seen at P18 or in adult animals.