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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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Related Experiment Video

Updated: Jun 27, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
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Single Extracellular VEsicle Nanoscopy.

Andras Saftics1, Sarah Abuelreich1, Eugenia Romano1

  • 1Department of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.

Journal of Extracellular Vesicles
|July 8, 2023
PubMed
Summary

We developed a new assay, Single Extracellular Vesicle Nanoscopy (SEVEN), to precisely analyze extracellular vesicle (EV) subpopulations. SEVEN can identify unique EV markers in pancreatic cancer patients, offering potential for new diagnostic tools.

Keywords:
SEVENextracellular vesicles (EVs)nanoscopypancreatic ductal adenocarcinoma (PDAC)quantitative single molecule localization microscopy (qSMLM)single EV analysis

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biomarker Discovery

Background:

  • Extracellular vesicles (EVs) are promising biomarkers, but isolating and characterizing their subpopulations remains challenging.
  • EVs are defined by tetraspanins (CD9, CD63, CD81) and source-specific markers.
  • Robust methods are needed for comprehensive EV subpopulation assessment.

Purpose of the Study:

  • To develop and validate a novel assay for comprehensive characterization of EV subpopulations.
  • To assess the utility of the assay in identifying disease-associated EV changes.
  • To explore the potential of EVs as biomarkers for pancreatic cancer.

Main Methods:

  • Combined affinity isolation with super-resolution imaging for EV analysis.
  • Developed the Single Extracellular Vesicle Nanoscopy (SEVEN) assay.
  • Quantified EV number, size, shape, and molecular content (tetraspanins).

Main Results:

  • SEVEN assay successfully quantified EV subpopulations from human plasma.
  • Detected a positive correlation between EV numbers and sample dilution.
  • SEVEN detected EVs from minimal sample volumes (∼0.1 μL).
  • Characterized size, shape, and tetraspanin content of CD9-, CD63-, and CD81-enriched EVs.
  • Identified distinct EV subpopulations in pancreatic cancer patients (e.g., smaller CD9-enriched EVs, larger IGF1R-enriched EVs).

Conclusions:

  • The SEVEN assay provides a validated method for detailed EV subpopulation characterization.
  • SEVEN demonstrates potential for identifying unique EV signatures associated with diseases like pancreatic cancer.
  • This platform can be advanced for characterizing both disease-associated and organ-associated EV subpopulations.