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Biological effect abundance analysis of hemolytic pathogens based on engineered biomimetic sensor
Qianqin Yuan1, Dongsheng Mao1, Xiaochen Tang2
1Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, PR China.
Biosensors & Bioelectronics
|July 9, 2023
Summary
This study introduces a novel biomimetic sensor using erythrocyte membranes and CRISPR-Cas12a to detect pathogen biological effects, not just physical presence. This method offers enhanced sensitivity and clinical potential for pathogen identification.
Area of Science:
- Biomimetic sensors
- CRISPR-Cas12a technology
- Pathogen detection
Background:
- Conventional methods detect pathogen physical abundance, not biological effects.
- Hemolytic pathogens pose a significant health risk.
- Need for sensitive and accurate pathogen detection methods.
Purpose of the Study:
- To develop a novel sensor for detecting the biological effects of hemolytic pathogens.
- To enhance detection sensitivity and accuracy compared to existing methods.
- To assess the clinical applicability of the developed sensor.
Main Methods:
- Constructed an erythrocyte membrane-encapsulated biomimetic sensor (EMS).
- Utilized the disruption of erythrocyte membranes by hemolytic pathogens for signal generation.
- Cascaded the EMS with CRISPR-Cas12a for signal amplification.
Main Results:
- Achieved over 6.67 × 10^4-fold improvement in detection sensitivity compared to traditional assays.
- Demonstrated sensitive response to pathogen pathogenicity changes.
- Obtained 95% accuracy in detecting simulated clinical samples.
Conclusions:
- The erythrocyte membrane-encapsulated biomimetic sensor cascaded with CRISPR-Cas12a (EMSCC) effectively detects pathogen biological effects.
- EMSCC offers superior sensitivity and pathogenicity responsiveness over PCR and ELISA.
- EMSCC shows significant potential for clinical applications in pathogen detection.
