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High-resolution Thermal Micro-imaging Using Europium Chelate Luminescent Coatings

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Cellular temperature probing using optically trapped single upconversion luminescence.

K Suresh¹, K Monisha¹, Aseefhali Bankapur¹

¹Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576104, India.

Analytica Chimica Acta

|July 9, 2023

Summary

This study investigated the temperature sensitivity of upconverting nanoparticles (UCNPs) for nanoscale temperature probing. Bare UCNPs showed higher thermal sensitivity than silica-coated or gold-coated UCNPs, especially within biological cells.

Keywords:

Cellular temperature probing Luminescence Optical trapping Plasmonic nanoparticles Thermal sensitivity Upconversion

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Area of Science:

Nanotechnology
Materials Science
Biophysics

Background:

Upconversion luminescence of rare earth element-doped nanoparticles (UCNPs) is researched for nanoscale temperature probing.
Low quantum efficiency and the role of surface modifications on temperature sensitivity remain challenges.
Investigating surface passivation and plasmonic particle effects on UCNP temperature sensitivity at the single-particle level is crucial.

Purpose of the Study:

To investigate the temperature sensitivity of bare UCNPs, UCNP@SiO2, and UCNP@SiO2@Au particles at the single-particle level.
To explore the influence of surface passivation (silica shell) and plasmonic particle incorporation (gold) on UCNP thermal sensitivity.
To assess the feasibility of using optically trapped UCNPs for intracellular temperature measurements.

Main Methods:

Optically trapping individual UCNPs (bare, UCNP@SiO2, UCNP@SiO2@Au) in a physiological temperature range (299 K–319 K).
Measuring luminescence from thermally coupled energy states to determine thermal sensitivity.
Performing in-situ temperature measurements within biological cells using optically trapped UCNPs.

Main Results:

Bare UCNPs exhibited higher thermal relative sensitivity than UCNP@SiO2 and UCNP@SiO2@Au in aqueous media.
Optically trapped UCNPs inside cells demonstrated increased absolute sensitivity with temperature.
Bare UCNPs showed greater thermal sensitivity compared to modified UCNPs within a biological cell environment.

Conclusions:

Single-particle level temperature measurement is achievable using optically trapped UCNPs.
Surface passivation and plasmonic particle incorporation can reduce the thermal sensitivity of UCNPs.
The thermal sensitivity of UCNPs is influenced by the surrounding environment, highlighting the importance of in-situ measurements within biological systems.