Composition of Sphagnum palustre L. extracts using different extraction methods.

Area of Science:

• Natural Product Chemistry
• Phytochemistry
• Green Extraction Technologies

Background:

• Sphagnum palustre L., a traditional Chinese herbal medicine, has limited research on its chemical constituents and bioactivities.
• Exploring novel extraction methods is crucial for unlocking the full potential of natural products like Sphagnum palustre.

Purpose of the Study:

• To investigate the chemical composition of Sphagnum palustre extracts.
• To evaluate the antibacterial and antioxidant capacities of these extracts.
• To assess the efficacy of deep eutectic solvents (DESs) in extracting active compounds from Sphagnum palustre.

Main Methods:

• Extraction of Sphagnum palustre L. phytosomes using conventional solvents (water, methanol, ethanol).
• Utilized choline chloride-based deep eutectic solvents (DESs) with hydrogen bond donors (citric acid, 1,2-propanediol) for enhanced extraction.
• Quantified total phenolic content (TPC) and identified major compounds using chromatographic techniques.

Main Results:

• Identified 253 compounds in Sphagnum palustre extracts, including citric acid, ethyl maltol, and thymol.
• The DES method combining 1,2-propanediol and choline chloride yielded the highest total phenolic content (39.02 ± 7.08 mg GAE/g DW).
• Demonstrated significant antibacterial and antioxidant capacities in the Sphagnum palustre extracts.

Conclusions:

• Deep eutectic solvents (DESs) are effective for extracting bioactive compounds from Sphagnum palustre.
• Sphagnum palustre possesses a rich chemical profile with potential applications in cosmetics and health products.
• This study validates the use of DESs for natural product extraction and highlights Sphagnum palustre as a valuable resource.