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An engineered biosensor enables dynamic aspartate measurements in living cells
Kristian Davidsen1,2, Jonathan S Marvin3, Abhi Aggarwal3
1Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.
Biorxiv : the Preprint Server for Biology
|July 10, 2023
Summary
Scientists developed a new GFP-based sensor (jAspSnFR3) to measure intracellular aspartate levels in mammalian cells. This tool enables real-time monitoring of aspartate, overcoming limitations of traditional methods for studying cell metabolism.
Area of Science:
- Biochemistry
- Cell Biology
- Metabolic Engineering
Background:
- Intracellular aspartate levels dynamically regulate mammalian cell function.
- Current methods like mass spectrometry for aspartate measurement lack throughput and temporal resolution.
- A need exists for advanced tools to comprehensively study aspartate metabolism.
Conclusions:
- jAspSnFR3 provides a robust, sensitive, and dynamic method for measuring intracellular aspartate.
- This novel sensor overcomes key limitations of traditional aspartate quantification techniques.
- jAspSnFR3 opens new avenues for high-throughput, real-time investigations into aspartate metabolism and its cellular roles.
