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Related Experiment Video

Updated: Jul 24, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Uncovering Protein Ensembles: Automated Multiconformer Model Building for X-ray Crystallography and Cryo-EM.

Stephanie A Wankowicz¹, Ashraya Ravikumar¹, Shivani Sharma^2,3

¹Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA, USA.

Biorxiv : the Preprint Server for Biology

|July 10, 2023

Summary

qFit software now better models biomolecular conformational heterogeneity. This automated strategy improves protein structural models derived from experimental data, aiding research into macromolecular dynamics and function.

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

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Last Updated: Jul 24, 2025

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps

Published on: July 19, 2024

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

Area of Science:

Structural Biology
Computational Biology
Biochemistry

Background:

Biomolecules exist in multiple conformational states essential for function.
Traditional methods like X-ray crystallography and cryo-electron microscopy (cryo-EM) yield ensemble-averaged data.
Current modeling often represents only a single conformation, failing to capture biomolecular complexity.

Conclusions:

qFit's automated approach reduces the difficulty of creating multiconformer models.
Facilitates the development of new hypotheses linking macromolecular dynamics to function.
Enables more accurate representation of biomolecular structures based on experimental data.