Related Experiment Video
Updated: Jul 24, 2025
09:26
Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro
Published on: June 6, 2025
373
Interaction between host G3BP and viral nucleocapsid protein regulates SARS-CoV-2 replication
Zemin Yang1, Bryan A Johnson2, Victoria A Meliopoulos3
1Department of Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Biorxiv : the Preprint Server for Biology
|July 10, 2023
Summary
The interaction between SARS-CoV-2 N protein and G3BP1 suppresses stress granule formation, promoting viral replication. Disrupting this interaction significantly reduces viral load and disease severity.
Area of Science:
- Virology
- Molecular Biology
- Structural Biology
Background:
- G3BP1 and G3BP2 are key proteins in stress granule formation during cellular stress and viral infections.
- The nucleocapsid (N) protein of SARS-CoV-2 interacts with G3BP1/2, but the functional impact is unknown.
Conclusions:
- The G3BP1-N protein interaction is critical for SARS-CoV-2 pathogenesis.
- This interaction promotes infection by enabling the N protein to inhibit G3BP1-mediated stress granule assembly.
- Targeting the G3BP1-N interaction could be a therapeutic strategy against SARS-CoV-2.
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