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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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Scanning Electron Microscopy01:07

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Electron Behavior00:54

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Electrons are negatively charged subatomic particles that are attracted to an orbit around the positively-charged nucleus of an atom. They reside in locations that are associated with energy levels called shells and are further organized into sub-shells and orbitals within each shell.
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Effects of EDTA on End-Point Detection Methods01:18

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

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Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over short...
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π Electron Effects on Chemical Shift: Overview01:27

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An applied magnetic field causes loosely bound π-electrons in organic molecules to circulate, producing a local or induced diamagnetic field over a large spatial volume. As the molecules tumble in solution, the field generated by π-electrons in spherical substituents results in a zero net field. However, the net field generated by π-electrons in non-spherical substituents is not zero. The effect of this induced field depends on the orientation of the molecule with respect to B0,...
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Updated: Jul 24, 2025

Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis
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Measurement of Total Calcium in Neurons by Electron Probe X-ray Microanalysis

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Electron-counting in MicroED.

Johan Hattne1,2, Max T B Clabbers2, Michael W Martynowycz1,2

  • 1Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095.

Biorxiv : the Preprint Server for Biology
|July 10, 2023
PubMed
Summary
This summary is machine-generated.

Electron-counting detectors offer faster, more accurate data collection for cryo-electron microscopy (cryo-EM) and MicroED, reducing radiation damage. Careful data collection is needed to manage coincidence loss for optimal results.

Keywords:
Cryo-EMElectron-countingMicroEDMicrocrystal electron diffraction

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Last Updated: Jul 24, 2025

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Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Electron-counting detectors provide high sensitivity and rapid readout capabilities.
  • These detectors are crucial for advancing cryogenic electron microscopy (cryo-EM) techniques.
  • Microcrystal electron diffraction (MicroED) of macromolecular crystals faces challenges with weak signals and radiation damage.

Conclusions:

  • Electron-counting detectors significantly enhance MicroED capabilities for macromolecular crystallography.
  • Careful data collection is essential to overcome limitations such as coincidence loss.
  • These detectors hold substantial promise for advancing structural biology research.