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miRNA326-5p Targets DKC1 Gene to Regulate Apoptosis-Related Proteins and Intervene in the Development of

Xiao-Hui Wang1, Shu-Feng Zhang1, Hai-Ying Wu2

  • 1Department of Pediatric Surgery, Henan Provincial People's Hospital, Zhengzhou 450000, China.

Analytical Cellular Pathology (Amsterdam)
|July 10, 2023
PubMed
Abstract

Insights

Congenital dyskeratosis 1 (DKC1) is highly expressed in neuroblastoma. Targeting DKC1 with miRNA326-5p inhibits neuroblastoma proliferation and promotes apoptosis by regulating apoptosis-related proteins.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Neuroblastoma (NB) is a pediatric cancer with complex genetic underpinnings.
  • The role of Congenital Dyskeratosis 1 (DKC1) in neuroblastoma development and progression requires further elucidation.
  • Understanding DKC1's regulatory mechanisms is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the effect of DKC1 on neuroblastoma.
  • To explore the regulatory mechanism of DKC1 in neuroblastoma.
  • To identify potential therapeutic targets for neuroblastoma treatment.

Main Methods:

  • DKC1 expression analysis in neuroblastoma using TCGA database and molecular assays.
  • In vitro studies involving siDKC1 transfection to assess effects on NB cell proliferation, cloning, metastasis, invasion, and apoptosis.
  • In vivo studies using a tumor-bearing mouse model with shDKC1 transfection to evaluate tumor growth and tissue changes.
  • Screening and identification of miRNA326-5p targeting DKC1 and subsequent functional assays.

Main Results:

  • DKC1 was found to be highly expressed in neuroblastoma cells and tissues.
  • DKC1 knockdown significantly decreased NB cell activity, proliferation, invasion, and migration, while increasing apoptosis.
  • miRNA326-5p was identified to target DKC1 mRNA, inhibiting its protein expression and consequently suppressing neuroblastoma cell proliferation and promoting apoptosis.

Conclusions:

  • DKC1 plays a significant role in promoting neuroblastoma proliferation and inhibiting apoptosis.
  • miRNA326-5p acts as a tumor suppressor by targeting DKC1, offering a potential therapeutic strategy for neuroblastoma.
  • The miRNA326-5p/DKC1 axis regulates apoptosis-related proteins, impacting neuroblastoma progression.

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