Artificial Downregulation of Ribosomal Protein L34 Restricts the Proliferation and Metastasis of Colorectal Cancer by

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Yi-Chao Liang1, Rui Li1, Shu-Rui Bao1

  • 1Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, P.R. China.

Human Gene Therapy
|July 10, 2023
PubMed

Insights

Ribosomal protein L34 (RPL34) promotes colorectal cancer (CRC) growth and metastasis by activating the JAK2/STAT3 pathway and inducing EMT. Its stability is regulated by CAND1, highlighting a new therapeutic target for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Aberrant expression of ribosomal protein L34 (RPL34) is implicated in various cancers.
  • The specific role of RPL34 in colorectal cancer (CRC) progression remained unclear.

Purpose of the Study:

  • To investigate the role of RPL34 in colorectal cancer (CRC) development and progression.
  • To identify molecular mechanisms underlying RPL34's function in CRC.

Main Methods:

  • Quantitative analysis of RPL34 expression in CRC tissues.
  • In vitro and in vivo assays to assess the impact of RPL34 manipulation on CRC cell behavior.
  • Western blotting, immunoprecipitation, and cell cycle analysis.
  • Investigation of the JAK2/STAT3 signaling pathway and epithelial-to-mesenchymal transition (EMT) markers.

Main Results:

  • RPL34 expression was significantly elevated in CRC tissues compared to normal tissues.
  • RPL34 overexpression enhanced CRC cell proliferation, migration, invasion, and metastasis.
  • RPL34 accelerated cell cycle, activated JAK2/STAT3 signaling, and induced EMT.
  • Cullin-associated NEDD8-dissociated protein 1 (CAND1) was identified as an RPL34 interactor, stabilizing RPL34 protein.
  • CAND1 overexpression promoted CRC phenotypes and EMT, effects rescued by RPL34 knockdown.

Conclusions:

  • RPL34 is a key driver of colorectal cancer (CRC) progression, promoting proliferation and metastasis.
  • RPL34 exerts its effects partly through activating the JAK2/STAT3 pathway and inducing EMT.
  • CAND1 stabilizes RPL34, contributing to CRC malignancy, suggesting a novel therapeutic target.
Keywords:
CAND1JAK2/STAT3 signaling pathwayRPL34colorectal cancerepithelial-to-mesenchymal transitionmetastasis

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