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Apta-sensor for selective determination of dopamine using chitosan-stabilized Prussian blue nanoparticles
Han Been Lee1, Seong Eun Son1, Gi Hun Seong1
1Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 426-791, South Korea. ghseong@hanyang.ac.kr.
Journal of Materials Chemistry. B
|July 10, 2023
Summary
Chitosan-stabilized Prussian blue nanoparticles create a new colorimetric assay for detecting dopamine. This aptamer-based sensor offers high sensitivity and reliability for clinical use.
Area of Science:
- Nanomaterials Science
- Analytical Chemistry
- Biomedical Engineering
Background:
- Dopamine (DA) detection is crucial for diagnosing neurological disorders.
- Existing detection methods often lack sensitivity or require complex procedures.
- Developing rapid, selective, and sensitive DA detection tools is essential for clinical applications.
Purpose of the Study:
- To fabricate chitosan-stabilized Prussian blue nanoparticles (CS/PBNPs).
- To develop a novel aptamer-based colorimetric assay for selective dopamine determination using CS/PBNPs.
- To evaluate the performance of the CS/PBNP apta-sensor for DA detection in human serum.
Main Methods:
- Fabrication of CS/PBNPs via a simple synthetic method.
- Characterization of CS/PBNPs using Scanning Electron Microscopy (SEM).
- Development of an aptamer-based colorimetric assay utilizing the peroxidase-like activity of CS/PBNPs for DA detection.
Main Results:
- CS/PBNPs exhibited uniform morphology (37.0 ± 3.2 nm) and strong peroxidase-like activity.
- The aptamer-based assay detected DA in a concentration range of 0.25–100 μM with a low limit of detection (LOD) of 0.16 μM.
- High recovery rates (92.6–103.9%) in spiked human serum confirmed the assay's reliability and reproducibility, outperforming a gold nanoparticle-based sensor.
Conclusions:
- CS/PBNPs serve as effective nanozymes for a sensitive colorimetric dopamine assay.
- The developed aptamer-based nanozyme system offers a simple, rapid, and highly sensitive method for DA detection.
- This assay holds significant potential for clinical diagnostics and applications due to its reliability and efficiency.
