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Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA in Situ Hybridization and

Markus A Grohme1, Olga Frank1, Jochen C Rink2

  • 1Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|July 10, 2023
PubMed

Keywords:
BrdUFormaldehyde fixationHigh-content fluorescence microscopyImmunocytochemistryMacerationPhospho-histone 3PlanarianRNA FISHTyramide signal amplification

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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