Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

First trimester chorionic villi sampling and direct chromosome preparations.

A T Tharapel, J V Dacus, S A Tharapel

    Clinical Genetics
    |June 1, 1986
    PubMed
    Summary

    Direct chromosome preparations from chorionic villi sampling are feasible. This method, used before pregnancy termination, yielded good quality chromosomes for genetic analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sustained rescue of prefrontal circuit dysfunction by antidepressant-induced spine formation.

    Science (New York, N.Y.)·2019
    Same author

    Gastric heterotopia in a gall bladder.

    Irish journal of medical science·2016
    Same author

    Genetic and treatment-related risk factors associated with external apical root resorption (EARR) concurrent with orthodontia.

    Orthodontics & craniofacial research·2015
    Same author

    Milnacipran for fibromyalgia.

    Issues in emerging health technologies·2008
    Same author

    Visual sex discrimination in goldfish: seasonal, sexual, and androgenic influences.

    Hormones and behavior·2004
    Same author

    Immunological and hematological effects observed in B6C3F1 mice exposed to JP-8 jet fuel for 14 days.

    Journal of toxicology and environmental health. Part A·2004

    Area of Science:

    • Prenatal diagnosis
    • Cytogenetics
    • Human genetics

    Background:

    • Chorionic villi sampling (CVS) is a prenatal diagnostic technique.
    • Obtaining direct chromosome preparations from CVS is challenging.
    • Accurate genetic information is crucial for reproductive decision-making.

    Purpose of the Study:

    • To evaluate the efficacy of direct chromosome preparations from chorionic villi.
    • To optimize hypotonic treatment and cell dispersal for CVS samples.
    • To assess the quality of G-banded chromosomes obtained from CVS.

    Main Methods:

    • Chorionic villi sampling performed on 52 patients undergoing elective pregnancy termination.
    • Direct chromosome preparations attempted on 42 successful villi samples.

    Related Experiment Videos

  • Hypotonic treatment using a 2:1 mixture of 0.075 M potassium chloride and 1% sodium citrate.
  • Cell dispersal achieved with 40% acetic acid.

    • Main Results:

    • Direct chromosome preparations were successful in 41 out of 42 villi samples.
    • Chromosomes exhibited good morphology.
    • G-banding analysis was successfully performed, yielding clear G-bands.

    Conclusions:

    • Direct chromosome preparations from chorionic villi are a viable method for genetic analysis.
    • The optimized hypotonic treatment and cell dispersal protocols enhance chromosome quality.
    • This technique provides reliable genetic information for prenatal diagnosis.