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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Dielectrophoresis-Assisted Self-Digitization Chip for High-Efficiency Single-Cell Analysis.

Yuling Qin1, Li Wu2, Daniel T Chiu3

  • 1School of Public Health, Nantong University, Nantong, Jiangsu, P. R. China. ylqin@ntu.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|July 10, 2023
PubMed
Summary

This study introduces a novel microfluidics chip using dielectrophoresis-assisted self-digitization (DEP-SD) for high-efficiency single-cell capture and analysis. This technology enables precise nucleic acid quantitation and genotyping for drug discovery and targeted cancer therapy.

Keywords:
Cell heterogeneityDielectrophoresisLoop-mediated isothermal amplification (LAMP)Self-digitization chipSingle-cell analysis

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Understanding cell population heterogeneity requires single-cell analysis of phenotypic information, including surface protein expression and nucleic acid content.
  • Existing methods for single-cell analysis face challenges in efficiency and precision for capturing and analyzing individual cells.

Purpose of the Study:

  • To design and demonstrate a dielectrophoresis-assisted self-digitization (DEP-SD) microfluidics chip for high-efficiency single-cell capture and in situ analysis.
  • To showcase the application of this platform for single-cell nucleic acid quantitation using loop-mediated isothermal amplification (LAMP).

Main Methods:

  • Development of a microfluidics chip that utilizes fluidic forces, interfacial tension, and channel geometry for spontaneous aqueous solution partitioning into microchambers.
  • Employing dielectrophoresis (DEP) with an applied AC voltage to guide and trap single cells at microchamber entrances.
  • Utilizing a process of flushing excess cells, releasing trapped cells, and sealing chambers with oil for in situ analysis.

Main Results:

  • The DEP-SD chip achieves high-efficiency single-cell capture and isolation within microchambers.
  • Demonstrated successful single-cell nucleic acid quantitation using loop-mediated isothermal amplification (LAMP) on the platform.
  • The platform enables precise analysis of single-cell genotypic information, such as cancer-related mutant genes.

Conclusions:

  • The DEP-SD microfluidics chip offers a powerful new tool for advancing single-cell research, particularly in drug discovery.
  • The ability to perform single-cell genotyping provides potential biomarkers for targeted cancer therapy.
  • This technology enhances the capacity for detailed analysis of cellular heterogeneity and function.