Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Introduction to Mechanisms of Enzyme Catalysis

Introduction to Mechanisms of Enzyme Catalysis

For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gas-phase recognition by a dopamine-selective cyclic tetrapeptide in the analysis of plant extracts.

Analytical methods : advancing methods and applications·2026

Same author

In situ iodine generation enables solution-phase polymerization of organic pollutants for continuous resource recovery from water.

Nature communications·2026

Same author

Oxygen-oxygen bond cleavage enables efficient photocatalytic H<sub>2</sub>O<sub>2</sub> production via an *O<sub>2</sub> dissociation pathway.

Nature communications·2026

Same author

Ce<sup>4+</sup>-Triggered Stable Pyrene Radical Cation Formation in a Hydrogen-Bonded Organic Framework Enabling on-Site Dual-Mode Sensing.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

A porous phosphate-rich β-cyclodextrin polymer for efficient and broad-spectrum enrichment of antibiotics.

Analytica chimica acta·2026

Same author

Cationic Covalent Organic Frameworks as a Solid-phase Microextraction Fiber Coating for the Rapid and Sensitive Determination of Legacy and Emerging Brominated Flame Retardants in Water.

Analytical chemistry·2026

Search research articles

Related Experiment Video

Updated: Jul 23, 2025

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Published on: July 10, 2017

Protocol for mechanochemistry-guided assembly strategy for enzyme encapsulation using covalent organic frameworks.

Rui Gao¹, Xiaoxue Kou¹, Rongwei He¹

¹MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

|July 11, 2023

Summary

This study introduces a new method for enzyme encapsulation using covalent organic frameworks, enhancing enzyme stability and reusability for biocatalysis applications.

Keywords:

Chemistry Material sciences X-ray Crystallography

More Related Videos

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Related Experiment Videos

Last Updated: Jul 23, 2025

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Published on: July 10, 2017

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Published on: April 8, 2020

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Area of Science:

Biochemistry
Materials Science
Chemical Engineering

Background:

Enzyme immobilization in porous frameworks improves enzyme stability and lifespan.
Developing efficient and robust enzyme encapsulation methods is crucial for biocatalysis.

Purpose of the Study:

To present a novel mechanochemistry-guided assembly strategy for enzyme encapsulation.
To detail a protocol for synthesizing and characterizing enzyme-loaded covalent organic frameworks (COFs).
To evaluate the biocatalytic activity and recyclability of encapsulated enzymes.

Main Methods:

Mechanochemical synthesis of covalent organic frameworks (COFs).
Enzyme loading quantification and material characterization techniques.
Assessment of enzyme biocatalytic activity and recyclability post-encapsulation.

Main Results:

Successful encapsulation of enzymes within de novo synthesized COFs.
Demonstration of enhanced enzyme stability and prolonged lifespan.
High biocatalytic activity and recyclability of the immobilized enzymes.

Conclusions:

Mechanochemistry offers a powerful approach for enzyme immobilization in COFs.
This strategy significantly enhances enzyme performance and reusability.
The developed protocol provides a robust method for creating advanced biocatalysts.