Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Special considerations while measuring oxygen saturation

Special considerations while measuring oxygen saturation

Assessing respiratory rate concurrently with pulse measurement is fundamental to patient care, providing valuable insights into the patient's respiratory function. The normal breathing rate for an adult usually falls within a normal range of 12 to 20 breaths per minute. Abnormal respiratory rates can signal underlying health conditions or the need for immediate intervention.
Ensuring accuracy in vital sign recordings while prioritizing patient comfort and minimizing anxiety is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Beyond component optimization: systems-level biodesign for lanthanide recovery.

Current opinion in biotechnology·2026

Same author

LambdaPy and LambdaR: Thermodynamics-Based Biogeochemical Reaction Modeling Packages for Integrating High-Resolution Mass Spectrometry Data.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Spatial and temporal metagenomics of river compartments reveals viral community dynamics in an urban impacted stream.

Frontiers in microbiomes·2026

Same author

Habitat Affinity of Riverine Dissolved Organic Matter Linked to Molecular Traits.

Global change biology·2026

Same author

Conserved environmental adaptations of stream microbiomes in the hyporheic zone across North America.

Microbiome·2025

Same author

Unveiling the dark matter of riverine dissolved organic matter and its role in molecular chemodiversity.

Water research·2025

Search research articles

Related Experiment Video

Updated: Jul 23, 2025

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

Metabolic multireactor: Practical considerations for using simple oxygen sensing optodes for high-throughput batch

Matthew H Kaufman¹, Joshua Torgeson², James C Stegen¹

¹Pacific Northwest National Laboratory, Earth and Biological Sciences Division, Richland, WA, United States of America.

|July 11, 2023

Summary

Researchers can now perform high-throughput, time-resolved oxygen consumption experiments on water and sediment samples using a cost-effective small batch reactor system. This approach enhances environmental research by enabling more comprehensive data collection from multiple samples simultaneously.

More Related Videos

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

Published on: October 26, 2021

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

Related Experiment Videos

Last Updated: Jul 23, 2025

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

High-Resolution Respirometry to Assess Bioenergetics in Cells and Tissues Using Chamber- and Plate-Based Respirometers

Published on: October 26, 2021

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

Area of Science:

Environmental Science
Analytical Chemistry
Biogeochemistry

Background:

Small batch reactor studies are crucial for understanding metabolic processes in aquatic and sediment environments.
Existing methods often lack the throughput and temporal resolution needed for comprehensive analysis.
High-quality, cost-effective experimental systems are needed to advance environmental research.

Purpose of the Study:

To present a novel system for conducting small batch reactor oxygen consumption experiments.
To provide practical guidance for constructing and operating a similar system for environmental research.
To enable high-throughput, high time-resolution data collection from water and sediment samples.

Main Methods:

Simultaneous measurement of oxygen concentrations in multiple small batch reactors.
Adaptation of established fluorescent dye-based oxygen sensing technology.
Detailed description of system construction, calibration, and operational procedures.

Main Results:

The system allows for simultaneous monitoring of oxygen levels across multiple reactors.
Achieves high throughput and high time-resolution data, overcoming limitations of previous studies.
Provides a practical, cost-effective solution for environmental metabolic studies.

Conclusions:

The presented system offers significant advantages for environmental research, including cost-effectiveness and high data quality.
It empowers researchers to conduct more in-depth metabolic studies with increased sample numbers and time points.
This work serves as a practical guide, minimizing challenges for others building similar experimental setups.