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Updated: Jul 23, 2025

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

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Published on: October 6, 2019

Microfluidic Bioreactor with Fibrous Micromixers for In Vitro mRNA Transcription.

Inseong Choi^1,2, Guk-Young Ahn^1,2, Eun Seo Kim^1,2

¹Biomedical and Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Bucheon-si, Gyeonggi-do 14662, Republic of Korea.

|July 12, 2023

Summary

A novel microfluidic bioreactor enables continuous messenger RNA (mRNA) production with efficient mixing. This platform demonstrates comparable mRNA quality to traditional methods, offering a powerful tool for microfluidic synthesis.

Keywords:

bioreactor in vitro transcription mRNA microfluidic micromixer

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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

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Last Updated: Jul 23, 2025

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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Area of Science:

Biotechnology
Chemical Engineering
Molecular Biology

Background:

Continuous manufacturing processes are increasingly important for biopharmaceutical production.
Microfluidic systems offer advantages in reaction control and efficiency.
Efficient mixing is crucial for optimizing reactions in microfluidic devices.

Purpose of the Study:

To develop and characterize a microfluidic bioreactor for continuous messenger RNA (mRNA) production.
To investigate the impact of fibrous micromixer design on mixing efficiency.
To evaluate the quality and performance of mRNA produced using the microfluidic system.

Main Methods:

Fabrication of a microfluidic bioreactor using electrospun microfibrous discs for micromixers.
Tuning microfiber diameter to optimize mixing performance.
Assessing mixing efficiency and mRNA synthesis via in vitro transcription.
Comparing the sequence and in vitro/in vivo performance of microfluidically produced mRNA with bulk-produced mRNA.

Main Results:

The microfluidic bioreactor incorporated fibrous micromixers and a macrochannel for continuous mRNA synthesis.
Micromixers with larger diameter fibrous microchannels achieved higher mixing efficiency, reaching 0.95 (complete mixing).
mRNA produced in the microfluidic bioreactor exhibited identical sequences and biological performance to conventionally produced mRNA.

Conclusions:

The developed microfluidic bioreactor with efficient fibrous micromixers is a viable platform for continuous mRNA production.
The system demonstrates high mixing efficiency and produces mRNA of comparable quality to bulk methods.
This technology holds potential for various microfluidic reaction applications.