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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

828
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
828
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

5.4K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.4K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

1.3K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.3K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

3.3K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
3.3K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K

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Related Experiment Video

Updated: Jul 23, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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ProSight Native: Defining Protein Complex Composition from Native Top-Down Mass Spectrometry Data.

Kenneth R Durbin1, Matthew T Robey1, Lilien N Voong1

  • 1Proteinaceous, Inc., Evanston, Illinois 60201, United States.

Journal of Proteome Research
|July 12, 2023
PubMed
Summary
This summary is machine-generated.

ProSight Native is a new software platform for analyzing native mass spectrometry data. It determines the complete composition of protein complexes, aiding structural biology research.

Keywords:
bioinformaticsdeconvolutionintact proteinnative mass spectrometryprotein complexprotein search enginetop down mass spectrometry

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Area of Science:

  • Structural Biology
  • Biochemistry
  • Computational Biology

Background:

  • Native mass spectrometry (MS) offers insights into protein complex composition.
  • Limited software tools exist for comprehensive analysis of native MS data, especially for intact complexes.

Purpose of the Study:

  • Introduce ProSight Native, an informatics platform for analyzing native protein and protein complex data.
  • Enable comprehensive determination of protein complex composition using native MS.

Main Methods:

  • Spectral deconvolution for mass determination.
  • Top-down database searching for protein identification.
  • Stoichiometry calculations for complex composition.
  • Tools for validating MS/MS fragment ions and mapping fragmentation data onto 3D structures.

Main Results:

  • ProSight Native successfully determined the composition of the homotetrameric Aquaporin Z membrane complex.
  • Deciphered the composition of a previously analyzed heterodimer complex with noncovalently bound ligands.
  • Developed new tools for fragment ion validation and structural mapping.

Conclusions:

  • ProSight Native provides a start-to-finish solution for native MS data analysis.
  • The platform reduces the informatics burden in native MS research.
  • ProSight Native facilitates broader application of native MS in structural biology.