Neuroanatomical asymmetry in the canine brain

The brains of humans and non-human primates exhibit left/right asymmetries in grey matter morphology, white matter connections, and functional responses. These asymmetries have been implicated in specialized behavioral adaptations such as language, tool use, and handedness. Left/right asymmetries are also observed in behavioral tendencies across the animal kingdom, suggesting a deep evolutionary origin for the neural mechanisms underlying lateralized behavior. However, it is still unclear to what extent brain asymmetries supporting lateralized behaviors are present in other large-brained animals outside the primate order. Canids and other carnivorans evolved large, complex brains independently and convergently with primates, and exhibit lateralized behaviors. Therefore, domestic dogs offer an opportunity to address this question. We examined T2-weighted MRI images of 62 dogs from 33 breeds, opportunistically collected from a veterinary MRI scanner from dogs who were referred for neurological examination but were not found to show any neuropathology. Volumetrically asymmetric regions of gray matter included portions of the temporal and frontal cortex, in addition to portions of the cerebellum, brainstem, and other subcortical regions. These results are consistent with the perspective that asymmetry may be a common feature underlying the evolution of complex brains and behavior across clades, and provide neuro-organizational information that is likely relevant to the growing field of canine behavioral neuroscience.