Related Experiment Video

Updated: Jul 23, 2025

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.8K

The DEAD-box RNA helicase Dbp5 is a key protein that couples multiple steps in gene expression

Luisa Querl1, Heike Krebber1

  • 1Abteilung für Molekulare Genetik, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften (GZMB), Georg-August Universität Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.

Biological Chemistry
|July 12, 2023
PubMed

Insights

The DEAD-box RNA helicase Dbp5 is crucial for cell viability, regulating mRNA export and translation. This study reveals Dbp5

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cell viability relies on the accurate export and translation of messenger RNA (mRNA).
  • Mature mRNAs are exported from the nucleus to the cytoplasm through the nuclear pore complex via the Mex67-Mtr2 complex.
  • The DEAD-box RNA helicase Dbp5 plays a role in the cytoplasmic displacement of export receptors.

Purpose of the Study:

  • To investigate the role of Dbp5 in cytoplasmic mRNA quality control pathways.
  • To determine the function of Dbp5 in translation termination.
  • To establish Dbp5 as a key regulator of mRNA expression.

Main Methods:

  • Analysis of mRNA export and translation processes.
  • Investigating the involvement of Dbp5 in cytoplasmic mRNA decay pathways.
  • Functional studies on Dbp5 in translation termination.

Main Results:

  • Dbp5 is implicated in cytoplasmic no-go and non-stop mRNA decay.
  • Dbp5 plays a critical role in translation termination.
  • Dbp5 acts as a master regulator of mRNA expression.

Conclusions:

  • Dbp5 is essential for multiple mRNA quality control steps, including export and translation.
  • The function of Dbp5 in translation termination highlights its significance in regulating gene expression.
  • Dbp5 is identified as a central regulator of mRNA expression, impacting cell viability.
Keywords:
Dbp5/DDX19NGDNSDmRNA exportmRNA quality controltranslation termination

More Related Videos

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.5K
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

2.7K

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
40
Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
28.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
945
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.1K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.7K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.3K