Geochemical mercury pools regulate diverse communities of hgcA microbes and MeHg levels in paddy soils

Rice paddies are unique artificial wetlands generating methylmercury (MeHg), a highly potent neurotoxin. However, the impact of diverse mercury (Hg) pools on the Hg-methylating communities during rice growth is unclear. This study investigates soil treated with five mercury forms (HgCl₂, α-HgS, β-HgS, nano-HgS, and Hg-DOM) at two levels (5 mg/kg and 50 mg/kg). The results showed a varying abundance of sulphate-reducing bacteria, Geobacteraceae, methanogens, and hgcA microbes in the soils across rice grown under different mercury treatments and concentrations. Soils treated with HgCl₂, nano-HgS and β-HgS had higher than average levels of hgcA-methanogen abundance, and the abundance significantly and positively correlated with MeHg concentration in all samples (p < 0.05). The shifting trends in Hg-methylating microbial structure following treatment with α-HgS, β-HgS, nano-HgS and Hg-DOM at both 5 and 50 mg/kg Hg levels were diverse compared with the control group. HgCl₂ treatment showed contrasting trends in community distribution of Hg methylators at 5 and 50 mg/kg Hg levels during rice growth. Dissolved organic carbon, redox potential and sulphate levels significantly correlated with variation in the Hg-methylating microbial community structure and MeHg production in soils.