MLF2 Negatively Regulates P53 and Promotes Colorectal Carcinogenesis

Related Experiment Video

Updated: Jul 23, 2025

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
28:15

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

12.4K

Debao Fang1,2, Hao Hu2, Kailiang Zhao1,2

  • 1Department of Thoracic Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 12, 2023
PubMed

Insights

Myeloid leukemia factor 2 (MLF2) negatively regulates the p53 pathway by inhibiting USP7 binding to p53, promoting colorectal cancer. MLF2 is elevated in colorectal cancer and linked to poor prognosis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • The p53 pathway is crucial for preventing cancer, and its inactivation is common in human malignancies.
  • Ubiquitin-specific protease 7 (USP7) is a key regulator of p53 stability through deubiquitination.

Purpose of the Study:

  • To identify novel regulators of the p53 pathway.
  • To investigate the role of myeloid leukemia factor 2 (MLF2) in p53 regulation and colorectal cancer.

Main Methods:

  • Co-immunoprecipitation assays to study protein interactions.
  • Western blotting to assess protein levels and stability.
  • Analysis of patient data to correlate MLF2 expression with colorectal cancer prognosis.

Main Results:

  • Myeloid leukemia factor 2 (MLF2) was identified as a novel negative regulator of p53.
  • MLF2 directly interacts with p53 and USP7, inhibiting USP7-mediated p53 deubiquitination and promoting p53 destabilization.
  • MLF2 expression is elevated in colorectal cancer tissues and associated with poor patient prognosis.
  • MLF2 plays an oncogenic role in colorectal cancer, partly through p53 suppression.

Conclusions:

  • MLF2 acts as a critical suppressor of p53 function.
  • The MLF2-p53 axis is implicated in the development and progression of colorectal cancer.
  • MLF2 represents a potential therapeutic target in colorectal cancer.
Keywords:
colorectal cancermyeloid leukemia factor 2 (MLF2)p53ubiquitin-specific protease 7 (USP7)

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.6K
Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer06:24

Author Spotlight: Liujunzi Decoction as a Traditional Chinese Treatment for Coloproctitis Cancer

Published on: October 13, 2023

970

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.9K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.2K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.5K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.3K