DNA Amplification Using Promega's PowerPlex^® Fusion Systems (5C and 6C)

Multiplex amplification and typing of short tandem repeat (STR) loci enable the rapid characterization of forensic samples for human identification purposes with the potential for high discriminatory power. Many commercial multiplex kits are available for consideration and purchase by DNA testing laboratories, including the PowerPlex^® Fusion 5C and PowerPlex^® Fusion 6C Systems offered by Promega Corporation. Herein we describe full-volume and half-volume amplification protocols utilizing extracted DNA for these respective multiplex kits, as well as procedures for direct amplification of lytic and nonlytic storage card punches and swabs.